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Updated: Jun 20, 2026

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples
Published on: September 28, 2022
[Purification of EGCG monomer from the extracts of tea by high speed countercurrent chromatography]
Yang Zhang1, Yan-Bin Jiang, Shao-Lie Huang
1School of Chemistry & Chemical Engineering, South China University of Technology, Guangzhou 510640, China.
Objective:
To investigate the feasibility and techniques for purifying epigallocatechin-3-gallate (EGCG) by high speed countercurrent chromatography (HSCCC).
Methods:
A HSCCC unit was used for the purification of EGCG from the extracts of tea.
Results:
The preferable solvent system was composed of Ether-Ethyl Acetate-H2O (4:10: 25, V/V), and the preferable technical conditions were as follows: the temperature was 35 degrees C, the rotation speed was 800 r/min and the mobile phase flow-rate was 2.5 mL/min.
Conclusion:
The purity of the obtained EGCG sample determined by high performance liquid chromatography (HPLC) is 95%.
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