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Updated: Jun 20, 2026
A Rat Model of Middle Cerebral Artery Occlusion/Reperfusion Without Damaging the Anatomical Structure of Cerebral Vessels
Published on: May 17, 2024
[Protective effect of 3'-methoxy puerarin on cerebral ischemic injury/ reperfusion in rats]
Jin Han1, Haitong Wan, Lijun Ge
1Traditional Chinese Medicine University of Zhejiang, Institute of Cardio-Cerebrovascular Diseases, Hangzhou 310053, China.
Objective:
To observe the effect of 3'-methoxy puerarin on cerebral infarction volume and free radical change of focal cerebral ischemia-reperfusion injury in rats and discuss the protective effect of 3'-methoxy puerarin on the cerebral ischemic/ reperfusion injury.
Method:
The thread method was used to induce middle cerebral artery embolization, to establish the focal cerebral ischemia-reperfusion model. Rats were divided into five groups: the sham and model group, the two-dose group (5, 10 mg x kg(-1) x d(-1)) of 3'-methoxy puerarin and nimodipine group (5 mg x kg(-1) x d(-1)). The behavior changes and volume of cerebral infarction were observed, and the leves of SOD and the content of MDA were measured.
Result:
3'-methoxy puerarin could significantly improve the symptoms of neurological deficit and reduce the infarct volume, and increased SOD activity and reduced the content of MDA of cortex in cerebral ischemia-reperfusion injury rat, the action of 10 mg x kg(-1) of 3'-methoxy puerarin is more remarkable.
Conclusion:
3'-methoxy puerarin has protective effect on focal cerebral ischemia-reperfusion injury in rat.
