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Updated: Jul 29, 2026

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Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
Published on: December 17, 2013
Flow cytometric detection of jellyfish venom induced cytotoxicity
J V Ordóñez1, H M Rubinstein, J W Burnett
1Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201.
Abstract:
Crude venoms of three poisonous jellyfish produce membrane depolarization as determined by the loss of fluorescence intensity of 3,3'-dipentyloxacarbocyanine iodide loaded cells measured by flow cytometry. This method for detecting jellyfish cytotoxicity was reproducible and more sensitive than mouse lethality assays.

