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Updated: Jun 20, 2026

Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Reversible effect of magnetic fields on human lymphocyte activation patterns: different sensitivity of naive and
Sergio Salerno1, Carmela La Mendola, Marco Pio La Manna
1Dipartimento di Biotecnologie Mediche e Medicina Legale, Università di Palermo, Palermo, Italy.
Abstract:
The aim of this study was to investigate the influence of 50 Hz magnetic or static magnetic fields of 0.5 mT on subsets of human CD4(+) T cells in terms of cytokine release/content, cell proliferation and intracellular free calcium concentration. CD4(+) T cells can be divided into different subsets on the basis of surface marker expression, such as CD45, and T cells can be divided into naive (CD45RA(+)) and memory (CD45RA(-)) cells. In this study, the effects of magnetic fields after 24 and 48 h of cell culture were analyzed. We found that the CD4(+)CD45RA(-) T subset were more sensitive after 2 h of exposure. Decreases in the release/content of IFN-gamma, in cell proliferation and in intracellular free calcium concentrations were observed in exposed CD4(+)CD45RA(-) T cells compared to CD4(+)CD45RA(+) T cells. The results suggest that exposure to the magnetic fields induces a delay in the response to stimulants and that modifications are rapidly reversible, at least after a short exposure.
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