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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Flow injection and sequential injection on-line pre-column derivatization for liquid chromatography
Rodjana Burakham1, Kate Grudpan
1Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Journal of Chromatographic Science
|September 24, 2009
Summary
This review covers on-line pre-column derivatization using flow injection (FI) and sequential injection (SI) for liquid chromatography (LC). These methods offer versatile applications for analyzing organic species and metal ions across various sample types.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- On-line pre-column derivatization is crucial for enhancing analyte detection in liquid chromatography (LC).
- Flow injection (FI) and sequential injection (SI) offer automated pre-treatment capabilities.
Purpose of the Study:
- To review on-line pre-column derivatization techniques using FI and SI for LC.
- To discuss the interfacing considerations of FI/SI systems with LC.
- To present diverse applications of these methods.
Main Methods:
- Review of existing literature on on-line derivatization with FI and SI.
- Discussion of practical aspects of integrating FI/SI systems with LC.
- Compilation of application examples.
Main Results:
- FI and SI are effective on-line pre-column derivatization strategies for LC.
- Successful applications demonstrated for organic species and metal ions.
- Interfacing FI/SI to LC requires careful consideration of system compatibility.
Conclusions:
- On-line derivatization using FI/SI provides a robust platform for LC analysis.
- The techniques are applicable to a wide range of sample matrices including biomedical, food, pharmaceutical, and environmental.
- Further advancements in FI/SI-LC are recommended for expanded analytical capabilities.
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