Related Experiment Video

Updated: Jun 20, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

High-dynamic-range heterodyne measurement of optical spectra

M O van Deventer, C M de Blok, C Park

    Optics Letters
    |September 24, 2009
    PubMed

    More Related Videos

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging05:45

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

    Published on: March 31, 2022

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging09:46

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

    Published on: April 28, 2022

    Related Concept Videos

    UV–Vis Spectrometers01:14

    UV–Vis Spectrometers

    The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

    When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
    IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

    IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

    A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
    According to Hooke's law, the vibrational frequency is directly proportional to the...
    Raman Spectroscopy Instrumentation: Overview01:26

    Raman Spectroscopy Instrumentation: Overview

    A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
    The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
    ¹H NMR: Interpreting Distorted and Overlapping Signals01:02

    ¹H NMR: Interpreting Distorted and Overlapping Signals

    Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
    As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
    UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

    UV–Vis Spectroscopy: Molecular Electronic Transitions

    In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...