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Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

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Related Experiment Video

Updated: Jun 20, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by ³²P-postlabeling

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Binding fullerenol C(60)(OH)(24) to dsDNA.

Mariana Pinteala¹, Andrei Dascalu, Cezar Ungurenasu

¹Petru Poni Institute of Macromolecular Chemistry, Aleea Grigore Ghica, 700487 Iasi, Romania.

International Journal of Nanomedicine

|September 24, 2009

Summary

Researchers report the first fullerene C(60)(OH)(24)-DNA complex, observing enhanced fluorescence proportional to DNA concentration. This discovery offers a sensitive method for DNA detection and characterization.

Keywords:

DNA complexation fluorescent probe fullerenol nanomedicine

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Last Updated: Jun 20, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by ³²P-postlabeling

Published on: March 20, 2018

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CD Spectroscopy to Study DNA-Protein Interactions

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Published on: February 10, 2022

Area of Science:

Nanotechnology
Biochemistry
Analytical Chemistry

Background:

Fullerenols, hydroxylated fullerenes, are explored for biomedical applications.
DNA detection methods are crucial for diagnostics and research.
Fluorescence-based assays offer high sensitivity.

Purpose of the Study:

To report the formation of the first C(60)(OH)(24)-DNA complex.
To investigate the fluorescence enhancement of fullerol C(60)(OH)(24) upon binding with DNA.
To establish a sensitive method for DNA quantification.

Main Methods:

Complexation of fullerol C(60)(OH)(24) with DNA.
Fluorescence spectroscopy to measure intensity changes.
Varying DNA concentrations to establish a calibration curve.
Determination of the detection limit.

Main Results:

The fluorescence intensity of fullerol C(60)(OH)(24) increased proportionally with DNA concentration (1 x 10(-9) to 8 x 10(-5) molL(-1)).
A low detection limit of 1.3 ng mL(-1) was achieved.
Fullerenol C(60)(OH)(24) demonstrated significant binding to the phosphate backbone of native dsDNA.
Binding also occurred with base-pairs within the major groove of sodium salt of dsDNA.

Conclusions:

A novel C(60)(OH)(24)-DNA complex exhibiting fluorescence enhancement has been synthesized.
This complex provides a sensitive and quantitative method for DNA detection.
The binding mechanism involves interactions with both the DNA backbone and base pairs.