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Biological wastewater treatment relies on the metabolic activity of microorganisms to remove pollutants from sewage. In modern treatment systems, this process is organized into sequential stages that progressively reduce solid material, dissolved organic matter, and microbial contamination. Each stage plays a distinct role in improving water quality and preparing the effluent for safe discharge or reuse.Primary and Secondary TreatmentPrimary treatment is a physical process that removes large...
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Related Experiment Video

Updated: Jun 20, 2026

Understanding Dissolved Organic Matter Biogeochemistry Through In Situ Nutrient Manipulations in Stream Ecosystems
09:38

Understanding Dissolved Organic Matter Biogeochemistry Through In Situ Nutrient Manipulations in Stream Ecosystems

Published on: October 29, 2016

Method development for measuring biodegradable dissolved organic nitrogen in treated wastewater.

Eakalak Khan1, Mayo Awobamise, Kimberly Jones

  • 1Department of Civil Engineering, North Dakota State University, Fargo, North Dakota, USA. eakalak.khan@ndsu.edu

Water Environment Research : a Research Publication of the Water Environment Federation
|September 25, 2009
PubMed
Summary

A new method accurately measures biodegradable dissolved organic nitrogen (BDON) in wastewater. This routine test uses established biochemical oxygen demand approaches for wastewater treatment plants (WWTPs).

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Last Updated: Jun 20, 2026

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Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Water Treatment Technology

Background:

  • Accurate measurement of biodegradable dissolved organic nitrogen (BDON) is crucial for optimizing wastewater treatment processes.
  • Existing methods may not be suitable for routine analysis at wastewater treatment plants (WWTPs).

Purpose of the Study:

  • To develop a reliable and routine method for determining BDON in treated wastewater.
  • To adapt established biochemical oxygen demand (BOD) and biodegradable dissolved organic carbon (BDOC) test principles for BDON analysis.

Main Methods:

  • The study investigated optimal inoculum type and concentration, and incubation periods.
  • Standard organic nitrogen solutions and filtered effluent from nutrient removal WWTPs were used for method validation.
  • Key parameters optimized included inoculum volume, concentration, and incubation duration.

Main Results:

  • The developed method achieved accurate and precise BDON results.
  • Optimal conditions involved using 2 mL of acclimated mixed-liquor suspended solids at 240 mg/L as inoculum.
  • An incubation period of 20 days was found to be effective, and post-incubation filtration was unnecessary.

Conclusions:

  • A robust and practical method for routine BDON determination in treated wastewater has been established.
  • The method's reliance on established BOD/BDOC principles facilitates its adoption in WWTPs.
  • This advancement aids in better monitoring and management of nitrogen in wastewater effluents.