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Absence of the c-Ha-ras codon 61 point mutation in murine solid tumors induced by subcutaneously applied
D F Andrews1, S J Collins, A L Reddy
1Department of Medicine, VA Medical Center, Seattle, WA 98108.
Abstract:
The c-Ha-ras-1 codon 61 A----T transversion is a highly consistent finding in murine skin DMBA-initiated/TPA-promoted papillomas and carcinomas. We performed Xba I RFLP analysis on DNA from clonal cell lines derived from 11 independent papillomas and a single carcinoma derived from topically applied DMBA and compared this to RFLP on DNA from 7 clonal fibrosarcoma cell lines derived from tumors arising after subcutaneous administration of the same carcinogen. The A----T point mutation was present in all of the papillomas and carcinoma derived by the classic DMBA-TPA protocol but was absent in all of the fibrosarcomas arising after subcutaneous administration of DMBA.