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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers
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Engineering surfaces for substrate-mediated gene delivery using recombinant proteins.

Jennifer C Rea1, Romie F Gibly, Nicolynn E Davis

  • 1Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, USA.

Biomacromolecules
|September 25, 2009
PubMed
Summary
This summary is machine-generated.

Recombinant proteins, like fibronectin fragments, can enhance gene delivery by improving DNA uptake and intracellular trafficking. These findings suggest bioactive sequences are key for optimizing gene transfer efficiency.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemistry

Background:

  • Immobilized natural proteins enhance substrate-mediated gene delivery.
  • Both bioactivity and physical properties of proteins contribute to gene delivery enhancement.

Purpose of the Study:

  • Investigate recombinant proteins as surface coatings for gene delivery.
  • Elucidate mechanisms of fibronectin-mediated gene transfer enhancement.

Main Methods:

  • Compared gene delivery using full-length fibronectin, fibronectin fragment (FNIII(7-10)), and a non-binding protein polymer (PP-12).
  • Assessed transgene expression, DNA association with cells, and intracellular trafficking for polyplexes and lipoplexes.

Main Results:

  • Full-length fibronectin yielded the highest transgene expression.
  • FNIII showed significantly higher expression than PP-12, indicating bioactive sequences enhance gene transfer.
  • FNIII enhanced intracellular trafficking and caveolar DNA levels for lipoplexes.

Conclusions:

  • Recombinant proteins can substitute for full-length extracellular matrix proteins in gene delivery.
  • Bioactive sequences within proteins play a crucial role in optimizing gene delivery steps.
  • Targeted use of recombinant proteins can maximize transfection efficiency.