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Peripheral blood mononuclear cell gene array profiles in patients with overactive bladder
Wellman Cheung1, Mark J Bluth, Christopher Johns
1SUNY Downstate Medical Center, Brooklyn, New York, USA.
Urology
|September 25, 2009
Summary
Peripheral blood mononuclear cells (PBMC) show altered gene expression in patients with overactive bladder (OAB). This suggests PBMC may serve as a diagnostic marker for OAB, aiding in understanding the condition.
Area of Science:
- Urology
- Genomics
- Immunology
Background:
- Overactive bladder (OAB) presents with symptoms like frequency and urgency, lacking a definitive diagnostic test.
- Current diagnostic methods for OAB may involve invasive procedures, highlighting the need for non-invasive markers.
- Previous research indicated peripheral blood mononuclear cells (PBMC) can function as reporters in other diseases.
Purpose of the Study:
- To evaluate the potential of peripheral blood mononuclear cells (PBMC) as a diagnostic marker for overactive bladder (OAB).
- To identify differentially regulated genes in PBMC from OAB patients compared to healthy controls.
Main Methods:
- Peripheral blood mononuclear cells (PBMC) were isolated from 21 patients diagnosed with OAB.
- RNA extracted from PBMC underwent microarray gene chip analysis to assess gene expression patterns.
Main Results:
- Microarray analysis identified 16 differentially regulated genes in PBMC from all OAB patients versus controls.
- Sex-based analysis revealed significant gene regulation differences: 74 in males and 30 in females.
- Specific genes like platelet-derived growth factors and tropomyosin were consistently downregulated in OAB patients.
Conclusions:
- Differential gene regulation in PBMC suggests their utility as a reporter for overactive bladder (OAB).
- PBMC analysis may offer a novel diagnostic marker for OAB.
- Identifying these genes could elucidate the underlying mechanisms of OAB and related tissue integrity issues.
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