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Updated: Jun 20, 2026
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Measuring Enzymatic Stability by Isothermal Titration Calorimetry
Published on: March 26, 2019
Thermal stability of bioactive enzymatic papers
Mohidus Samad Khan1, Xu Li, Wei Shen
1Australian Pulp and Paper Institute, Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.
Colloids and Surfaces. B, Biointerfaces
|September 25, 2009
Summary
Enzymes like alkaline phosphatase (ALP) and horseradish peroxidase (HRP) adsorbed on paper show significantly enhanced thermal stability. This paper-based enzyme immobilization is ideal for creating cost-effective, high-volume bioactive surfaces.
Area of Science:
- Biotechnology
- Materials Science
- Enzyme Engineering
Background:
- Enzyme immobilization is crucial for industrial applications.
- Paper offers a promising, low-cost substrate for enzyme immobilization.
- Understanding the thermal stability of immobilized enzymes is key to their practical use.
Purpose of the Study:
- To evaluate the thermal stability of alkaline phosphatase (ALP) and horseradish peroxidase (HRP) immobilized on paper.
- To compare the stability of paper-adsorbed enzymes with enzymes in solution.
- To explore the potential of paper and inkjet printing for creating bioactive surfaces.
Main Methods:
- Enzymes (ALP and HRP) were adsorbed onto paper.
- Thermal stability was assessed using a colorimetric technique measuring product complex intensity.
- Enzyme degradation kinetics were analyzed.
Main Results:
- Paper-adsorbed ALP and HRP retained functionality and selectivity.
- Immobilization on paper increased enzyme thermal stability by 2-3 orders of magnitude.
- Half-lives at 23°C were 533 h for ALP and 239 h for HRP.
- Thermal degradation followed two sequential first-order reactions.
Conclusions:
- Paper is an excellent material for immobilizing enzymes, significantly enhancing their thermal stability.
- Inkjet printing enables the low-cost, high-volume production of bioactive paper surfaces.
- This approach is suitable for manufacturing robust, enzyme-based diagnostic or industrial tools.
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