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Related Experiment Video

Updated: Jun 20, 2026

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

Published on: December 17, 2015

Chromatin flow cytometry identifies changes in epigenetic cell states.

Nadine Obier¹, Albrecht M Müller

¹Institute for Medical Radiation and Cell Research, University of Würzburg, Germany.

Cells, Tissues, Organs

|September 25, 2009

Summary

We developed flow cytometry protocols to measure histone modifications in single cells. This technique rapidly analyzes chromatin marks and epigenetic changes in cell populations.

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Area of Science:

Epigenetics
Cell Biology
Biochemistry

Background:

Histone modifications are crucial regulators of gene expression.
Analyzing these marks at a single-cell level provides deeper biological insights.
Current methods can be time-consuming or lack single-cell resolution.

Purpose of the Study:

To establish and validate flow cytometry protocols for quantifying histone modifications.
To enable simultaneous analysis of chromatin marks with other cellular features.
To demonstrate the utility of this method in studying epigenetic changes.

Main Methods:

Development of quantitative flow cytometry protocols.
Application to mouse embryonic stem cells.
Analysis of histone acetylation and methylation levels.

Main Results:

Successfully quantified diverse histone modifications on a single-cell basis.
Demonstrated rapid, simultaneous analysis of chromatin marks.
Identified changes in epigenetic marks in response to drug treatment and differentiation.

Conclusions:

Chromatin flow cytometry is a powerful tool for single-cell epigenetics.
This method facilitates the study of dynamic changes in histone modifications.
Enables high-throughput analysis of epigenetic states in various biological contexts.