Related Experiment Video

Updated: May 5, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

8.9K

The expanding universe of p53 targets

Daniel Menendez1, Alberto Inga, Michael A Resnick

  • 1Laboratory of Molecular Genetics, National Institute of Environmental Health Science, Research Triangle Park, North Carolina 27709, USA.

Nature Reviews. Cancer
|September 25, 2009
PubMed

Insights

The p53 tumor suppressor protein regulates genes critical for cancer. This review details its response elements (REs), including non-canonical ones, expanding understanding of p53

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • The p53 tumor suppressor protein is a master regulator of the cellular response to stress.
  • Mutations or altered expression of p53 are implicated in the majority of human cancers.
  • p53 exerts its function by binding to specific DNA sequences known as response elements (REs).

Purpose of the Study:

  • To review the identification and function of p53 target response elements (REs).
  • To highlight the significance of non-canonical REs in the p53 regulatory network.
  • To provide a comprehensive overview of how p53 REs contribute to cancer etiology.

Main Methods:

  • Literature review of studies on p53 target REs.
  • Analysis of canonical and non-canonical p53 RE sequences.
  • Discussion of factors influencing p53 transactivation and repression.

Main Results:

  • p53 regulates hundreds of genes through sequence-specific DNA binding.
  • Canonical REs are well-characterized, but non-canonical REs expand the regulatory landscape.
  • p53 levels, cofactors, and RE sequence variations modulate gene regulation.

Conclusions:

  • Understanding p53 REs, including non-canonical forms, is crucial for comprehending p53's role in cancer.
  • The diversity of p53 REs contributes to the complexity of p53-mediated gene regulation.
  • Further research into non-canonical REs may reveal novel therapeutic targets in cancer treatment.

More Related Videos

Purification of Ubiquitinated p53 Proteins from Mammalian Cells10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.6K
Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

371

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.2K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
8.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.0K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
4.7K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
2.4K