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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Related Experiment Video

Updated: Jun 20, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Imaging objects hidden in scattering media using a fluorescence-absorption technique.

K M Yoo, Z W Zang, S A Ahmed

    Optics Letters
    |September 25, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A new imaging method improves visualization of hidden objects in scattering materials by using luminescence and spectral filtering. This technique enhances image quality for better detection in challenging environments.

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    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
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    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Materials Science

    Background:

    • Imaging through scattering media is challenging due to light diffusion.
    • Conventional methods struggle to resolve objects obscured by dense materials.

    Purpose of the Study:

    • To introduce a novel imaging technique for enhanced visualization of hidden objects.
    • To improve image quality in highly scattering environments.

    Main Methods:

    • Object luminescence is induced.
    • Luminescent light is selected for imaging, filtering out illumination light.
    • Spectral filtering targets luminescence portions strongly absorbed by the medium.

    Main Results:

    • Significantly enhanced image quality for hidden objects.
    • Improved resolution and contrast in scattering media.
    • Demonstrated effectiveness of spectral selection for noise reduction.

    Conclusions:

    • The novel luminescence-based imaging technique offers superior performance.
    • Spectral filtering is a key factor in achieving high-quality images.
    • This method has potential applications in various scientific and industrial fields.