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A Microfluidic Chip for ICPMS Sample Introduction
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Microfluidic HPLC-Chip devices with integral channels containing methylstyrenic-based monolithic media.

Karla M Robotti1, Hongfeng Yin, Reid Brennen

  • 1Agilent Technologies, Inc., Santa Clara, CA 95051, USA. karla_robotti@agilent.com

Journal of Separation Science
|September 25, 2009
PubMed
Summary

New poly(methylstyrene-bis-p-vinylphenyl)ethane (MS/BVPE) stationary phases in HPLC-Chip devices show comparable protein separation to commercial media. This adaptable material offers excellent repeatability for advanced analytical workflows.

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Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • High-performance liquid chromatography (HPLC) chip devices offer miniaturized separation capabilities.
  • Developing novel stationary phases is crucial for enhancing HPLC-Chip performance and versatility.

Purpose of the Study:

  • To prepare and evaluate poly(methylstyrene-bis-p-vinylphenyl)ethane (MS/BVPE) as a stationary phase in polyimide HPLC-Chip devices.
  • To compare the chromatographic performance of MS/BVPE monoliths against conventional particulate media.

Main Methods:

  • Thermally initiated free radical polymerization was used to synthesize the MS/BVPE stationary phase within polyimide microfluidic devices.
  • The devices were coupled to UV and mass spectrometry (MS) detectors for analysis.
  • Side-by-side comparisons were conducted with commercial HPLC-Chip devices using particulate media.

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Last Updated: Jun 20, 2026

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Main Results:

  • MS/BVPE monoliths demonstrated chromatographic behavior comparable to particulate media for protein separations, with similar peak widths and separation times.
  • Controlled porosity through polymerization time allowed for potential application in small analyte (< 500 Da) separations.
  • Excellent run-to-run (≤ 0.16% RSD) and chip-to-chip (1.4% RSD) reproducibility was achieved due to the monolith's mechanical stability.

Conclusions:

  • MS/BVPE is a viable alternative separation medium for HPLC-Chip devices, offering robust performance for protein analysis.
  • The material's tunable porosity and mechanical stability suggest potential for broader applications, including small molecule analysis and complex sample preparation workflows.