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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

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Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

Desorption electrospray ionization: achieving rapid sampling rates.

Griffin K Barbula1, Matthew D Robbins, Oh Kyu Yoon

  • 1Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.

Analytical Chemistry
|September 26, 2009
PubMed
Summary
This summary is machine-generated.

Desorption electrospray ionization (DESI) coupled with Hadamard transform time-of-flight mass spectrometry (HTTOFMS) achieves high sampling rates for surface imaging. This novel approach enhances chemical analysis speed and spatial resolution.

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Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
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Published on: February 19, 2020

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06:21

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10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Surface Science

Background:

  • Desorption electrospray ionization (DESI) is a versatile ambient ionization technique for mass spectrometry.
  • Improving the sampling rate and imaging capabilities of DESI is crucial for rapid chemical analysis.
  • Existing DESI methods face limitations in temporal resolution and throughput for complex surface analysis.

Purpose of the Study:

  • To investigate the sampling rate and imaging capabilities of DESI using a rotating sample platform.
  • To explore the application of Hadamard transform time-of-flight mass spectrometry (HTTOFMS) for enhanced DESI analysis.
  • To establish a method for high-speed DESI surface imaging with improved signal-to-noise ratio.

Main Methods:

  • Utilized a rotating sample platform with DESI-compatible dyes on poly(methyl methacrylate) discs.
  • Employed Hadamard transform time-of-flight mass spectrometry (HTTOFMS) for millisecond acquisition of full mass spectra.
  • Controlled disk rotation rate to adjust analyte residence time and sampling rate, achieving spectral resolution up to 80 samples/s.

Main Results:

  • Achieved spectral resolution of alternating analytes up to 80 samples/s and single-analyte spots up to 50 samples/s.
  • Demonstrated high DESI solution flow rates without compromising chemical information integrity due to rapid surface movement.
  • Showcased additive combination of data from multiple rotations for enhanced signal-to-noise chemical imaging of surfaces.

Conclusions:

  • The combination of a rotating sample platform and HTTOFMS significantly enhances DESI sampling rates and imaging capabilities.
  • Multipass data analysis allows for detailed examination of analyte signal dynamics, defining temporal resolution limits.
  • This multipass analysis approach is proposed as a powerful method for advanced DESI surface imaging applications.