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Updated: Jun 20, 2026

Rapid Generation of Amyloid from Native Proteins In vitro
Published on: December 5, 2013
Diversity of kinetic pathways in amyloid fibril formation
Giovanni Bellesia1, Joan-Emma Shea
1Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, USA. gbellesia@chem.ucsb.edu
Abstract:
The kinetics of peptide oligomerization was investigated using Langevin Dynamics simulations and a coarse-grained peptide model. The simulations show a rich diversity of aggregation pathways, modulated by the beta-sheet propensity (flexibility) of the peptide. Aggregation into amyloidlike fibrils occurs via three main mechanisms: (i) formation of fibrils directly from the assembly of early ordered oligomers, (ii) fibril formation via the formation of on-pathway, nonfibrillar aggregates high in beta-sheet content, and (iii) formation of amorphous aggregates followed by reorganization to beta-sheet aggregates and to fibrils. beta-sheet, nonfibrillar aggregates also appeared as long-lived, "off-pathway" end-product species.
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