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[Ginsenoside Rg3 induces apoptosis of human lung squamous cell carcinoma SK-MES-1 cell line]
Xin Wang1, Yu-ling Zheng, Ke Li
1Department of Oncology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. wxgoal@163.com
Objective:
To investigate the effect of ginsenoside Rg3 on the apoptosis and survivin expression in human lung squamous cell carcinoma cell line SK-MES-1.
Methods:
SK-MES-1 cells were divided into Rg3 treatment group, blank control group and positive control (arsenic trioxide) group. The apoptotic rate of the cells in each group was determined using flow cytometry, and the expression of survivin protein and mRNA was detected by immunocytochemistry and RT-PCR, respectively.
Results:
A 48-h treatment with Ginsenoside Rg3 induced increased apoptotic rate of SK-MES-1 cells in a dose-dependent manner. Ginsenoside Rg3 significantly downregulated the expressions of survivin protein and mRNA as compared with the expression levels in the blank control group (P<0.05).
Conclusion:
Ginsenoside Rg3 can induce the apoptosis of SK-MES-1 cells, the mechanism of which may involve inhibited survivin expression.
Insights
Ginsenoside Rg3 promotes apoptosis in lung cancer cells by reducing survivin expression. This natural compound offers a potential therapeutic strategy for lung squamous cell carcinoma.
Area of Science:
- Oncology
- Molecular Biology
- Pharmacology
Background:
- Lung squamous cell carcinoma (LSCC) is a major subtype of non-small cell lung cancer.
- Survivin is an anti-apoptotic protein often overexpressed in cancers, including LSCC.
- Ginsenoside Rg3 is a natural compound derived from ginseng with potential anti-cancer properties.
Purpose of the Study:
- To evaluate the impact of ginsenoside Rg3 on apoptosis in the human LSCC cell line SK-MES-1.
- To investigate the effect of ginsenoside Rg3 on survivin protein and mRNA expression in SK-MES-1 cells.
Main Methods:
- SK-MES-1 cells were treated with ginsenoside Rg3, with blank and arsenic trioxide controls.
- Apoptotic rates were quantified using flow cytometry.
- Survivin protein and mRNA levels were assessed via immunocytochemistry and RT-PCR, respectively.
Main Results:
- Ginsenoside Rg3 treatment dose-dependently increased the apoptotic rate of SK-MES-1 cells after 48 hours.
- Significant downregulation of both survivin protein and mRNA was observed in ginsenoside Rg3-treated cells compared to controls (P<0.05).
Conclusions:
- Ginsenoside Rg3 effectively induces apoptosis in human lung squamous cell carcinoma SK-MES-1 cells.
- The mechanism underlying ginsenoside Rg3-induced apoptosis may involve the inhibition of survivin expression.