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Updated: Jun 20, 2026

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
Published on: November 12, 2016
[High-performance capillary electrophoresis for determining caffeic acid content in compound Yinhuangjiedu decoction]
Objective:
To establish a method for determining the content of caffeic acid in compound Yinhuangjiedu decoction using high-performance capillary electrophoresis (HPCE).
Methods:
The optimized HPCE for determining caffeic acid content utilized a fused-silica capillary tube (75 microm x 60 cm, effective length of 53 cm) with 20 mmol/L borate as the running buffer (pH=9.18), a constant voltage of 12 kV, a sampling time of 5 s at 25 degrees celsius, and UV detection wavelength at 313 nm.
Results:
The linear range of caffeic acid was 20-100 microg/ml.
Conclusion:
HPCE is simple, rapid, and sensitive for quality control of the compound Yinhuangjiedu decoction.
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