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Cystitis increases colorectal afferent sensitivity in the mouse.

Pablo Rodolfo Brumovsky1, Bin Feng, Linjing Xu

  • 1Center for Pain Research, Departments of Anesthesiology, University of Pittsburgh, Pennsylvania, USA. brumovskypr@upmc.edu

American Journal of Physiology. Gastrointestinal and Liver Physiology
|September 26, 2009
PubMed
Summary

Cyclophosphamide (CYP) induced cystitis in mice increased colorectal afferent sensitivity to mechanical stimuli. This suggests a peripheral mechanism contributes to cross-organ hypersensitivity between the bladder and colon.

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Area of Science:

  • Neuroscience
  • Gastroenterology
  • Urology

Background:

  • Colon inflammation can cause bladder hypersensitivity, and cystitis can cause colon hypersensitivity.
  • These phenomena are known as cross-organ sensitization.
  • A peripheral mechanism for this cross-organ sensitization is investigated.

Purpose of the Study:

  • To determine if cystitis alters the sensitivity of pelvic nerve colorectal afferents.
  • To investigate the peripheral mechanisms underlying cross-organ sensitization between the bladder and colon.

Main Methods:

  • Male C57BL/6 mice were treated with cyclophosphamide (CYP) to induce cystitis or saline.
  • Mechanosensitive properties of colorectal afferent fibers were studied using an in vitro preparation.
  • Afferent fibers were exposed to an inflammatory soup (IS) to assess sensitization.

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Main Results:

  • CYP treatment increased the number of colorectal stretch-sensitive fibers excited by IS.
  • CYP treatment increased the proportion of chemosensitive colorectal afferents.
  • Bladder afferent responses to IS were decreased in chemosensitivity in CYP-treated mice.

Conclusions:

  • CYP-induced cystitis enhances the mechanical sensitivity of colorectal afferents.
  • These findings support a peripheral contribution to cross-organ sensitization in pelvic organs.
  • Altered afferent sensitivity in one organ impacts the sensitivity of another.