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Updated: Jun 20, 2026

Intravitreous Injection for Establishing Ocular Diseases Model

Intravitreous Injection for Establishing Ocular Diseases Model

Published on: October 1, 2007

Experimental model of intravitreal injection techniques.

Jean-Pierre Hubschman¹, Robert E Coffee, Jean-Louis Bourges

¹David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. hubschman@jsei.ucla.edu

Retina (Philadelphia, Pa.)

|September 26, 2009

Summary

Using 30-gauge needles and deep intravitreal (IVT) injections can minimize drug reflux and vitreous incarceration. This technique may enhance therapeutic outcomes and reduce severe complications like endophthalmitis.

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Area of Science:

Ophthalmology
Surgical Techniques
Drug Delivery

Background:

Intravitreal injections (IVT) are crucial for treating various ocular diseases.
Minimizing drug reflux and vitreous incarceration is essential for maximizing therapeutic efficacy and patient safety.

Purpose of the Study:

To evaluate drug reflux and vitreous leakage after different intravitreal injection techniques in porcine eyes.
To compare the impact of needle size and injection depth on these outcomes.

Main Methods:

Quantified drug reflux using methylene blue in 150 porcine eyes.
Assessed vitreous incarceration using balanced salt solution (BSS) in 150 porcine eyes.
Examined effects of 27, 30, and 32-gauge needles, injection depth, and speed using an ocular endoscope.

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Intravitreous Injection for Establishing Ocular Diseases Model

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Main Results:

Thirty-gauge needles showed significantly less drug reflux compared to 32-gauge and 27-gauge needles (P < 0.01).
Thirty-two-gauge needles demonstrated less vitreous incarceration (P < 0.01), though all tracts showed internal incarceration.
Deep IVT injections reduced reflux but did not alter vitreous incarceration.

Conclusions:

Employing 30-gauge needles and deep needle tip placement during IVT injections may reduce reflux and vitreous incarceration.
These optimized techniques could enhance therapeutic effects and decrease severe complications like retinal detachment and endophthalmitis.