Related Experiment Video
Updated: Jun 20, 2026
Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle
Published on: June 8, 2022
Assessment of lipids in skeletal muscle by LCModel and AMARES
Jan Weis1, Lars Johansson, Francisco Ortiz-Nieto
1Department of Radiology, Uppsala University Hospital, Uppsala, Sweden. jan.weis@radiol.uu.se
Purpose:
To process single voxel spectra of the human skeletal muscle by using an advanced method for accurate, robust, and efficient spectral fitting (AMARES) and by linear combination of model spectra (LCModel). To determine absolute concentrations of extra- (EMCL) and intramyocellular lipids (IMCL).
Materials And Methods:
Single-voxel proton magnetic resonance spectroscopy (PRESS) was used to obtain the spectra of the calf muscles. Unsuppressed water line was used as a concentration reference. A new prior knowledge for AMARES was proposed to estimate the concentrations of EMCL and IMCL. The prior knowledge was derived from the spectrum of vegetable oil. The results were compared with the values estimated by LCModel. Absolute concentrations of total lipid content in millimoles per kilogram wet weight were used for the comparisons.
Results:
Absolute concentrations of total lipid content in skeletal muscle were estimated by AMARES and LCModel. Very good correlation of the total fat (EMCL + IMCL) and IMCL concentrations was achieved between both data processing approaches.
Conclusion:
Assessment the absolute concentrations of muscular lipids by AMARES and LCModel can be performed with comparable accuracy.
