Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Why physicians underuse patient-reported outcomes in atopic dermatitis and chronic urticaria - Insights from the UCARE/ADCARE PROMUSE study.

The World Allergy Organization journal·2026
Same author

Skin physiology during daylight photodynamic therapy with additional fractional laser therapy.

European journal of dermatology : EJD·2026
Same author

Molecular Diagnostics in Hand Dermatoses: Clinical Findings and Health-Related Quality of Life in a 3-Year Follow-Up Cohort Study.

Dermatology and therapy·2026
Same author

Unmet Needs in Treatment Escalation for Chronic Spontaneous Urticaria: Findings From the CURE Registry.

Allergy·2026
Same author

Molecular Classification in a Cohort of Occupational Dermatological Patients: Diagnostic Results and Course of Ability to Work and Sick Leave Over Two Years.

Acta dermato-venereologica·2026
Same author

Strengths and Limitations of Sodium Lauryl Sulfate as an Irritant Control in Patch Testing.

Contact dermatitis·2025

Related Experiment Video

Updated: Jun 20, 2026

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response
06:31

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response

Published on: October 3, 2019

Quantitative patch and repeated open application testing in hydroxyisohexyl 3-cyclohexene carboxaldehyde

Axel Schnuch1, Wolfgang Uter, Heinrich Dickel

  • 1Information Network of Departments of Dermatology, University of Göttingen, Göttingen/Germany. aschnuch@med.uni-goettingen.de

Contact Dermatitis
|September 29, 2009
PubMed
Summary
This summary is machine-generated.

To prevent allergic reactions, the safe concentration of hydroxyisohexyl 3-cyclohexene carboxaldehyde (HICC) in products for sensitized individuals is below 0.027%. This research helps establish safe usage levels for HICC in cosmetics.

More Related Videos

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
09:39

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Published on: January 27, 2014

Related Experiment Videos

Last Updated: Jun 20, 2026

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response
06:31

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response

Published on: October 3, 2019

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
09:39

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Published on: January 27, 2014

Area of Science:

  • Dermatology
  • Toxicology
  • Cosmetic Science

Background:

  • Hydroxyisohexyl 3-cyclohexene carboxaldehyde (HICC) is a common fragrance ingredient.
  • Allergic contact dermatitis is a significant concern with fragrance compounds.
  • Establishing safe concentration limits for sensitized individuals is crucial for consumer safety.

Purpose of the Study:

  • To determine the maximum concentration of HICC that does not elicit an allergic reaction in patients with known sensitization.
  • To provide data for setting safe usage levels of HICC in cosmetic products.

Main Methods:

  • Repeated Open Application Testing (ROAT) on 64 sensitized subjects using HICC in perfume and cream formulations at varying concentrations (0.005-2.5%).
  • Confirmatory patch testing with HICC preparations at 2.5% and 5% concentrations.
  • Statistical analysis to correlate ROAT results with patch test sensitivity and specificity.

Main Results:

  • Estimated tolerable HICC concentrations for 90% of sensitized individuals were <88.2 ppm for cream and <270 ppm for perfume.
  • Patch test sensitivity ranged from 88.5-98.1%, with specificity from 37.5-87.5%.
  • A significant inverse correlation was found between ROAT concentrations and patch test reaction strength, indicating varying susceptibility levels.

Conclusions:

  • Recommended use concentrations to protect 90% of sensitized individuals range from 0.009% to 0.027%, varying by product type.
  • Avoiding excessive HICC concentrations is essential to prevent further sensitization.
  • Continuous monitoring is necessary to validate the effectiveness of HICC exposure recommendations.