A practical glucose biosensor based on Fe(3)O(4) nanoparticles and chitosan/nafion composite film

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biomedical Engineering

Background:

• Glucose biosensors are crucial for diabetes management.
• Existing biosensors often face challenges with sensitivity and interference.
• Iron oxide nanoparticles (Fe3O4 NPs) offer catalytic activity, while nafion films provide anti-interference properties.

Purpose of the Study:

• To develop a practical and highly sensitive glucose biosensor.
• To leverage the peroxidase-like activity of Fe3O4 NPs and the anti-interference capability of nafion.
• To create a stable and reliable platform for glucose detection.

Main Methods:

• Fabrication of a glucose biosensor by immobilizing glucose oxidase (GOD) with Fe3O4 NPs on a platinum electrode using chitosan.
• Cross-linking the enzyme-immobilized layer with glutaraldehyde.
• Coating the modified electrode with a thin nafion film.

Main Results:

• The developed biosensor exhibited high sensitivity (11.54 µAcm⁻²mM⁻¹) and a low detection limit (6 x 10⁻⁶ M).
• A wide linear range (6 x 10⁻⁶ to 2.2 x 10⁻³ M) was achieved with good storage stability.
• The nafion film effectively minimized interference, enabling accurate glucose detection in human serum samples.

Conclusions:

• The combined use of Fe3O4 NPs and nafion film offers a promising strategy for developing practical glucose biosensors.
• The fabricated biosensor demonstrates excellent performance characteristics, including sensitivity, stability, and anti-interference.
• This approach holds potential for simple, optimized glucose monitoring in clinical applications.