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Updated: Jun 20, 2026
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Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
Published on: October 26, 2009
Concurrent extraction of proteins and RNA from cell-laden hydrogel scaffold free of polysaccharide interference
Chunming Wang1, Xin Li, Yongchang Yao
1Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, N1.3-B2-13, Singapore 637457, Republic of Singapore.
Abstract:
Concurrent extractions of proteins and RNA from cell-laden scaffolds would be of great help to biomaterialists and tissue engineers. Here we describe a procedure to extract proteins from the discard solution generated during the RNA isolation from polysaccharide-rich hydrogel scaffolds. This approach allows to obtain proteins and RNA from same sample while eliminating the polysaccharide interference.
