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Updated: Jun 20, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Immunoassay of low to moderately abundant anionic proteins utilizing selective immobilization to chitosan-coated
Karen J Rees-Milton1, Tassos P Anastassiades
1Division of Rheumatology and Arthritis Centre, Department of Medicine, Queen's University, Kingston, ON K7L3N6, Canada. reesmilton@sympatico.ca
Abstract:
The quantitation of low to moderately abundant serum proteins is a common problem encountered in biochemistry. A physical property of the antigen of interest needs to be exploited in the initial binding step of an immunoassay resulting in capture (purification). We describe a two-stage immunoassay utilizing chromogenic and chemiluminescent substrates which is applied to two serum anionic proteins. In this assay, anionic serum proteins are selectively bound to positively charged chitosan-coated polystyrene plates at pH 6.1, in the presence of detergent. The assay has a detection limit of 0.1 microg/mL and a 1000-fold range.
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