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Isolation of a lectin and a galactoxyloglucan from Mucuna sloanei seeds
Daniele M A Teixeira-Sá1, Fany Reicher, Renata C Braga
1Laboratório de Lectinas e Glicoconjugados, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 6020, CEP 60451-980 Fortaleza, CE, Brazil. d_teixeira@uece.br
Abstract:
A lectin and a galactoxyloglucan were characterized from Mucuna sloanei seed cotyledons. The galactoxyloglucan, isolated by water extraction and ethanol precipitation, had Glc:Xyl:Gal proportions in a molar ratio of 1.8:1.7:1.0 and a molar mass (M(w)) of 1.6x10(6)g mol(-1). The lectin (sloanin), isolated from the same seed by affinity chromatography on cross-linked Adenanthera pavonina galactomannan, gave two protein bands by SDS-PAGE (36 and 34 kDa) and one peak by gel filtration (63.6 kDa). Its N-terminal sequence indicated approximately 69% identity with soybean agglutinin to leguminous lectins. Circular dichroism (CD) spectra established that sloanin predominantly contains beta-sheet structures. Sloanin has approximately 5.5% carbohydrate and displayed hemagglutinating activity against rabbit and enzyme treated human erythrocytes, inhibited only by D-Gal containing sugars. The interaction between sloanin and storage cell-wall galactoxyloglucan was tested by affinity chromatography and fluorescence spectroscopy.
