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Updated: Jun 20, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

Tritrichomonas fetus extracellular products decrease progressive motility of bull sperm.

C M Ribeiro¹, M B Falleiros, S D Bicudo

¹Department of Veterinary Hygiene and Public Health, Faculty of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil.

|September 29, 2009

Summary

Tritrichomonas fetus parasite products harm bovine sperm, reducing motility and causing cellular damage. This parasite

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Analysis of Epididymal Protein Synthesis and Secretion

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Published on: August 25, 2018

Area of Science:

Veterinary Science
Parasitology
Reproductive Biology

Background:

Tritrichomonas fetus is a protozoan parasite known to cause infertility and abortion in cattle.
Limited information exists on the susceptibility of bovine sperm to T. fetus and its effects.

Purpose of the Study:

To investigate the in vitro interaction between T. fetus and bovine sperm.
To assess the impact of T. fetus extracellular products on sperm function and viability.

Main Methods:

In vitro incubation of bovine sperm with T. fetus.
Reverse transcription-polymerase chain reaction (RT-PCR) to detect gene expression.
Computer-assisted semen analysis (CASA) for motility assessment.
Annexin-V/propidium iodide assay for viability and cellular damage evaluation.

Main Results:

Sperm adhered to T. fetus, leading to cell agglutination.
T. fetus continuously expressed its cysteine peptidase gene.
Extracellular products significantly reduced sperm progressive motility.
While not causing immediate death, parasite products increased indicators of sperm cellular damage.

Conclusions:

Extracellular products from T. fetus exhibit cytotoxicity towards bovine sperm.
These products decrease sperm progressive motility and induce cellular damage.
This cytotoxic effect may contribute to T. fetus-induced infertility in cattle.