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Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview

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Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
08:48

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Enhanced electro-mediated gene delivery using carrier genes.

Jeong Ah Kim1, Won Gu Lee, Neon Cheol Jung

  • 1School of Chemical and Biological Engineering, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151-742, South Korea. jakim2002@hotmail.com

Bioelectrochemistry (Amsterdam, Netherlands)
|September 29, 2009
PubMed
Summary
This summary is machine-generated.

Using carrier genes with electroporation significantly enhances gene delivery efficiency in cells. This method boosts target gene expression, offering a promising approach for clinical gene therapy applications.

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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Gene Therapy

Background:

  • Low transfection and expression of target genes hinder clinical gene delivery.
  • Electroporation is a common method for gene delivery, but efficiency can be limited.

Purpose of the Study:

  • To enhance gene transfection efficiency using carrier genes during electroporation.
  • To evaluate the impact of carrier genes on target gene expression in HeLa cells.

Main Methods:

  • HeLa cells were transfected with luciferase genes (pGL3-control) using electroporation.
  • Prokaryotic expression vector pCR2.1 was employed as a carrier gene.
  • Luciferase activity was measured to quantify transfection efficiency.

Main Results:

  • Carrier genes significantly improved the transfer of luciferase genes into the cell membrane.
  • Luciferase activity increased two- to three-fold in the presence of carrier genes.
  • No significant correlation was found between the weight ratio of genes and transfection efficiency.

Conclusions:

  • Carrier genes are effective in enhancing gene transfection and expression via electroporation.
  • This strategy holds potential for improving gene delivery in various biological applications.
  • Further research may explore optimal carrier gene types and delivery conditions.