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Published on: January 11, 2018
Blue orange light emission from biogenic synthesized silver nanoparticles using Trichoderma viride
Mohammed Fayaz1, C S Tiwary, P T Kalaichelvan
1CAS in Botany, University of Madras, Guindy Campus, Chennai 600 025, India. nanofayaz@gmail.com
Colloids and Surfaces. B, Biointerfaces
|September 29, 2009
Summary
Researchers synthesized stable, water-soluble silver nanoparticles (AgNPs) using a biological route with Trichoderma viride filtrate. These biocompatible AgNPs exhibit fluorescence properties, offering advantages over traditional dyes for potential labeling applications.
Area of Science:
- Nanomaterials Science
- Biotechnology
- Biophysics
Background:
- Noble metal-based nanomaterials offer advantages as fluorescence labels due to their water solubility and biocompatibility.
- Conjugating noble metals with biomolecules creates novel fluorescence probes with enhanced properties compared to conventional dyes.
Purpose of the Study:
- To synthesize and characterize silver nanoparticles (AgNPs) using a biological method.
- To investigate the fluorescence properties of biologically synthesized AgNPs.
- To explore the potential of these AgNPs as fluorescence labels.
Main Methods:
- Biosynthesis of silver nanoparticles (2-4 nm) using filtrate of Trichoderma viride.
- Characterization using UV-vis spectrophotometry, FTIR, High-Resolution Transmission Electron Microscopy (HrTEM), Energy-Dispersive X-ray spectroscopy (EDX), X-ray Diffraction (XRD), and fluorescence spectroscopy.
Main Results:
- Stable silver hydrosol formation observed upon reduction of silver ions by Trichoderma viride filtrate.
- UV-vis spectrum showed maximum absorbance at 405 nm.
- FTIR confirmed the presence of proteins, HrTEM revealed monodispersed spherical nanoparticles, and EDX/XRD confirmed elemental silver.
- Synthesized AgNPs exhibited fluorescence emission between 320-520 nm.
Conclusions:
- Biologically synthesized silver nanoparticles are stable, monodispersed, and possess fluorescence properties.
- These nanoparticles demonstrate potential as biocompatible and water-soluble fluorescence labels.
- The biological route offers an efficient method for producing functional silver nanoparticles.
