Massilia jejuensis sp. nov. and Naxibacter suwonensis sp. nov., isolated from air samples

Two Gram-negative, motile, rod-shaped bacteria (strains 5317J-18T and 5414S-25T) were isolated from air samples collected in the Jeju Island and Suwon region of Korea, respectively. Phylogenetically, strain 5317J-18T was grouped with the genus Massilia with Massilia brevitalea byr23-80T as the closest relative (98.8% sequence similarity). Strain 5414S-25T was affiliated with the genus Naxibacter with Naxibacter haematophilus CCUG 38318T as the closest relative (98.8% sequence similarity). The mean DNA-DNA relatedness values between strain 5317J-18T and M. brevitalea DSM 18925T and Massilia aurea DSM 18055T were 43 and 36%, respectively. The mean DNA-DNA hybridization values between strain 5414S-25T and N. haematophilus KACC 13771T, M. brevitalea DSM 18925T, Massilia timonae DSM 16850T, Naxibacter varians KACC 13770T, M. aurea DSM 18055T, Massilia lutea DSM 17473T and Massilia albidiflava DSM 17472T ranged from 33 to 42%. Both novel strains had ubiquinone Q-8 as the predominant isoprenoid quinone and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1 omega7c) and C16:0 as the major fatty acids. Both strains also showed similar polar lipid profiles with phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. The DNA G+C contents of strains 5317J-18T and 5414S-25T were 66.1 and 67.8%, respectively. On the basis of their phenotypic, chemotaxonomic and genotypic characteristics, the new strains represent novel species in the genera Massilia and Naxibacter. Strain 5317J-18T (=KACC 12634T=DSM 21309T) is proposed as the type strain of Massilia jejuensis sp. nov. and strain 5414S-25T (=KACC 12635T=DSM 21311T) is proposed as the type strain of Naxibacter suwonensis sp. nov.