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Related Concept Videos

Bacterial Protein Maturation01:26

Bacterial Protein Maturation

Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...

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Updated: Jun 20, 2026

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis
06:14

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis

Published on: September 26, 2025

Protein turnover in mycobacterial proteomics.

Prahlad K Rao1, Qingbo Li

  • 1Center for Pharmaceutical Biotechnology, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60607, USA. rkrish4@uic.edu

Molecules (Basel, Switzerland)
|September 29, 2009
PubMed
Summary

Protein turnover analysis offers new insights into Mycobacterium tuberculosis biology, especially for dormant cells. This method reveals subtle protein changes missed by traditional proteomic studies, aiding drug discovery for tuberculosis.

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Last Updated: Jun 20, 2026

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis
06:14

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis

Published on: September 26, 2025

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
08:48

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

Published on: July 11, 2025

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
07:56

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Published on: September 3, 2012

Area of Science:

  • Microbiology
  • Systems Biology
  • Biochemistry

Background:

  • Mycobacterium tuberculosis (M. tuberculosis) biology is crucial for tuberculosis research.
  • Systems biology approaches have advanced understanding of M. tuberculosis pathogenesis.
  • M. tuberculosis exhibits environmental modulation and dormancy, posing research challenges.

Purpose of the Study:

  • To review M. tuberculosis proteome responses to stress.
  • To compare protein abundance studies with protein turnover studies.
  • To highlight the utility of protein turnover analysis for exploring M. tuberculosis proteome dynamics.

Main Methods:

  • Review of existing proteomic and transcriptomic data.
  • Analysis of protein abundance versus protein turnover data.
  • Focus on M. tuberculosis responses to various stresses.

Main Results:

  • Conventional proteomic studies struggle with dormant M. tuberculosis.
  • Discrepancies exist between proteome and transcriptome data.
  • Protein turnover analysis reveals subtle changes in protein synthesis, degradation, and secretion.

Conclusions:

  • Protein turnover analysis provides a more comprehensive view of the M. tuberculosis proteome.
  • This approach is particularly valuable for studying dormant or persistent M. tuberculosis cells.
  • Turnover studies can aid in tuberculosis drug discovery and understanding proteome dynamics.