Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Smart biomaterials: From responsiveness to closed-loop sensing and feedback.

Trends in biotechnology·2026
Same author

Molecular endotypes in musculoskeletal disorders: a systematic review.

BMC musculoskeletal disorders·2026
Same author

Association of metformin with osteoarthritis progression and total joint arthroplasty: evidence from the UK Biobank, a large population-based cohort study.

Clinical rheumatology·2026
Same author

The rise and fall of TRPV1-targeted analgesia in osteoarthritis: a critical appraisal.

Expert opinion on pharmacotherapy·2026
Same author

Next-generation therapies for osteoarthritis: the evolving role of cell therapy products.

Experimental & molecular medicine·2026
Same author

Disruption of the microenvironmental ecosystem in subchondral bone marrow lesions: Roles in osteoarthritis pathophysiology, pain and progression.

Osteoarthritis imaging·2026

Related Experiment Video

Updated: Jun 20, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and

Neil S Graham1, Abigail L Clutterbuck, Nicholas James

  • 1Nottingham Arabidopsis Stock Centre, Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough, UK.

Veterinary Journal (London, England : 1997)
|September 30, 2009
PubMed
Summary

This study shows that using genomic DNA (gDNA)-based probe selection on human Affymetrix GeneChip arrays effectively analyzes the equine transcriptome. This method allows for detailed study of gene expression in horses, a species with limited prior research.

More Related Videos

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
09:04

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Related Experiment Videos

Last Updated: Jun 20, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
09:04

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Area of Science:

  • Genomics
  • Transcriptomics
  • Bioinformatics

Background:

  • Affymetrix GeneChip arrays are established tools for transcriptome profiling across various species.
  • A novel genomic DNA (gDNA)-based probe selection method expands the applicability of GeneChips to new species.
  • The equine transcriptome has historically received limited research attention.

Purpose of the Study:

  • To evaluate the efficacy of gDNA-based probe selection on the Affymetrix U133+2 GeneChip array for equine transcriptome analysis.
  • To explore gene expression patterns in key equine tissues and cell types.

Main Methods:

  • Utilized Affymetrix U133+2 GeneChip arrays with gDNA-based probe selection.
  • Analyzed transcriptomes from equine brain, liver, and primary equine articular chondrocytes.
  • Performed gene ontology analysis on differentially expressed genes.

Main Results:

  • Successfully detected over 29,000 transcripts in equine brain, liver, and chondrocytes.
  • Gene ontology analysis revealed expected functional categories within each tissue type.
  • Correlated gene expression levels with tissue-specific functions and phenotypes.

Conclusions:

  • gDNA-based probe selection on human GeneChip arrays is a viable method for equine transcriptome studies.
  • This approach enables comprehensive analysis of the equine transcriptome, revealing tissue-specific gene expression patterns.
  • The findings open new avenues for research into equine biology and disease.