Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Central and Peripheral Alterations of Retinal and Choroidal Vasculature in Multiple Sclerosis: Insights from Multimodal Imaging.

Ophthalmology science·2026
Same author

CD11c+ CD8 T cells cause IFN-γ-dependent autoimmune neuroinflammation that is restrained by PD-1 signaling.

JCI insight·2026
Same author

Th17 effector cytokines induce shared and distinct microglial and endothelial cell responses in post-streptococcal encephalitis.

bioRxiv : the preprint server for biology·2026
Same author

Astrocyte-derived extracellular vesicles as antigen-specific therapy for neuromyelitis optica spectrum disorder in the mouse model.

bioRxiv : the preprint server for biology·2026
Same author

Retraction of Adult neural stem cells expressing IL-10 confer potent immunomodulation and remyelination in experimental autoimmune encephalitis.

The Journal of clinical investigation·2025
Same author

GM-CSF production by immune cells in steady state and autoimmune neuroinflammation mapped using fate reporting mice.

Frontiers in immunology·2025

Related Experiment Video

Updated: Jun 20, 2026

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Differential effect of IL-27 on developing versus committed Th17 cells.

Mohamed El-behi1, Bogoljub Ciric, Shuo Yu

  • 1Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|September 30, 2009
PubMed
Summary

Interleukin-27 (IL-27) inhibits the development of new Th17 cells but has minimal impact on already differentiated Th17 cells, suggesting limited therapeutic use in established Th17-driven diseases.

More Related Videos

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
07:46

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Related Experiment Videos

Last Updated: Jun 20, 2026

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
12:59

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
07:46

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Interleukin-27 (IL-27) is known to inhibit the differentiation of naive CD4(+) T cells into Th17 cells.
  • The effect of IL-27 on established, differentiated Th17 cells remains largely uncharacterized.
  • Understanding IL-27's regulation of committed Th17 cells is crucial for evaluating its therapeutic potential in Th17-associated inflammatory diseases.

Purpose of the Study:

  • To investigate the effect of IL-27 on committed Th17 cells, including memory and encephalitogenic Th17 cells.
  • To determine if IL-27 can suppress the function and phenotype of already differentiated Th17 cells.
  • To assess the therapeutic implications of IL-27's effects on established Th17 responses.

Main Methods:

  • Assessing IL-27 receptor (IL-27R) expression on committed Th17 cells.
  • Measuring retinoid-related orphan receptor (ROR) gamma t and RORalpha expression in IL-27 treated Th17 cells.
  • Evaluating IL-17A production by reactivated Th17 cells in the presence or absence of IL-27.
  • Conducting adoptive transfer experiments of experimental autoimmune encephalomyelitis (EAE) with IL-27 treated Th17 cells.
  • Performing ex vivo analysis of Th17 cell phenotype in spleen and CNS.

Main Results:

  • IL-27 had minimal effect on committed Th17 cells, despite functional IL-27R expression.
  • IL-27 did not suppress RORgamma t or RORalpha expression in committed Th17 cells.
  • IL-27 did not affect the frequency or cytokine secretion of committed Th17 cells.
  • Both in vivo-derived memory Th17 cells and CNS-infiltrating encephalitogenic Th17 cells produced similar IL-17A levels with or without IL-27.
  • IL-27 failed to suppress the encephalitogenicity of Th17 cells in an EAE adoptive transfer model.
  • Transferred Th17 cells maintained their phenotype regardless of IL-27 treatment.

Conclusions:

  • IL-27 exhibits limited efficacy in suppressing established Th17 cell responses.
  • Unlike its effect on de novo differentiation, IL-27 has little to no impact on committed Th17 cells.
  • Therapeutic applications of IL-27 may have restricted benefits in inflammatory conditions with pre-existing aggressive Th17 responses.