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Related Concept Videos

pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Proofreading01:43

Proofreading

Synthesis of new DNA molecules starts when DNA polymerase links nucleotides together in a sequence that is complementary to the template DNA strand. DNA polymerase has a higher affinity for the correct base to ensure fidelity in DNA replication. The DNA polymerase furthermore proofreads during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.Errors during Replication Are Corrected by the DNA Polymerase EnzymeGenomic DNA is synthesized in...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
PCR01:32

PCR

Overview
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
The Scientific Method01:32

The Scientific Method

The scientific method is a detailed, empirical problem-solving process used by biologists and other scientists. This iterative approach involves formulating a question based on observation, developing a testable potential explanation for the observation (called a hypothesis), making and testing predictions based on the hypothesis, and using the findings to create new hypotheses and predictions.Generally, predictions are tested using carefully-designed experiments. Based on the outcome of these...

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Related Experiment Video

Updated: Jun 20, 2026

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

How a submitted manuscript is processed.

W C G Peh1, K H Ng

  • 1Singapore Medical Journal, 2 College Road, Singapore 169850. smj.editor@sma.org.sg

Singapore Medical Journal
|September 30, 2009
PubMed
Summary
This summary is machine-generated.

Manuscript processing involves editors, peer reviewers, and copy editors to ensure quality and adherence to journal standards. Understanding the editorial office checklist helps authors navigate this complex publication process efficiently.

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Area of Science:

  • Scholarly Publishing
  • Scientific Communication

Background:

  • Manuscript processing is a critical, multi-stage procedure in scholarly publishing.
  • Authors often face challenges understanding the intricacies of journal workflows.

Purpose of the Study:

  • To elucidate the key components and roles within the manuscript processing system.
  • To inform authors about essential elements of the editorial office checklist.

Main Methods:

  • Review of standard operating procedures in academic journal publishing.
  • Analysis of the functions performed by editorial staff, peer reviewers, and copy editors.

Main Results:

  • Manuscript processing requires coordination between authors, editors, and reviewers.
  • Copy editors play a vital role in standardizing manuscripts to journal style and enhancing readability.

Conclusions:

  • Awareness of the editorial checklist and the roles of involved personnel streamlines the publication process.
  • Effective collaboration ensures the integrity and clarity of published scientific work.