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Related Experiment Videos

Mouse opsin. Gene structure and molecular basis of multiple transcripts.

M R al-Ubaidi1, S J Pittler, M S Champagne

  • 1Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030.

The Journal of Biological Chemistry
|November 25, 1990
PubMed
Summary

Mouse opsin gene transcripts vary in size and expression. This complexity arises from selective use of polyadenylation sites, not alternative splicing, affecting the 3' untranslated region.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Neuroscience

Background:

  • The mouse opsin gene generates multiple mRNA transcripts of varying sizes.
  • These transcripts are detectable early in development and are translated in vivo.
  • Understanding the molecular basis of this transcript heterogeneity is crucial.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying the complex transcription pattern of the mouse opsin gene.
  • To characterize the genomic structure of the mouse opsin gene and its flanking regions.

Main Methods:

  • Genomic DNA characterization of the mouse opsin gene and flanking regions.
  • Northern hybridization using exon- and intron-specific probes.
  • Polymerase chain reaction (PCR) analysis of opsin cDNA.

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Main Results:

  • Transcription initiates at a single site.
  • Transcript variants are not due to alternative splicing.
  • Multiple polyadenylation signals at the 3' end of the gene were identified.
  • Selective use of these polyadenylation sites generates transcripts differing in 3'-untranslated region length.

Conclusions:

  • The complex opsin mRNA transcript pattern in mice is primarily due to alternative polyadenylation.
  • Similar transcript heterogeneity is observed in other species, including rat, human, and frog.
  • This finding provides insight into gene expression regulation in opsin and potentially other genes.