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Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
Published on: November 2, 2013
Expression of mRNA for human type-I LHRH receptor transcript forms in human benign prostatic hyperplasia
Bernadett Rózsa1, Aliz Juhász, Andrea Treszl
1Department of Biopharmacy, School of Pharmacy, University of Debrecen, 4032 Debrecen, Hungary.
International Journal of Oncology
|September 30, 2009
Summary
Researchers found luteinizing hormone-releasing hormone receptor-I (LHRH-R-I) mRNA in human benign prostatic hyperplasia (BPH) tissue. This suggests LHRH-R-I may have multiple transcript forms relevant to BPH.
Area of Science:
- Endocrinology
- Molecular Biology
- Urology
Background:
- Vertebrates possess four luteinizing hormone-releasing hormone (LHRH) isoforms and one LHRH receptor (LHRH-R).
- The human genome contains LHRH-I and LHRH-II genes, with LHRH-I comprising four exons and three introns.
- Three LHRH receptor or receptor-like genes exist, including the well-established type-I LHRH receptor (LHRH-R-I) gene, which has three exons and two introns.
Purpose of the Study:
- To investigate the expression of LHRH-R-I transcript forms in human benign prostatic hyperplasia (BPH).
- To determine the presence and potential variants of LHRH-R-I mRNA in BPH tissue using reverse transcriptase-polymerase chain reaction (RT-PCR).
Main Methods:
- Thirty-five human BPH specimens were obtained from surgical procedures.
- Normal human pituitaries served as control tissue.
- RNA extraction followed by RT-PCR using gene-specific primers for LHRH-R-I transcript forms (F1/R1, F2/R3, F1'/R2') was performed.
Main Results:
- LHRH-R-I mRNA transcripts (F1/R1, 319 bp) were detected in 60% (21/35) of BPH samples.
- LHRH-R-I mRNA transcripts (F2/R3, 309 bp) were found in 14% (5/35) of BPH samples.
- No LHRH-R-I mRNA transcripts (F1'/R2', 219 bp) were detected in the BPH specimens.
Conclusions:
- mRNA for LHRH-R-I was successfully detected in human BPH specimens.
- The results indicate that the LHRH-R-I gene may exhibit more than two splice variants or uncharacterized transcript forms.
- Further research into the expression of LHRH-R-I and its transcript forms in human BPH is warranted.
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