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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
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An optical-manipulation technique for cells in physiological flows.

Hu Zhang, Neng H Chen, Alicia El Haj

    Journal of Biological Physics
    |September 30, 2009
    PubMed
    Summary

    Researchers developed a new method using optical tweezers to precisely manipulate human red blood cells (RBCs) in fluid flow, minimizing cell stress. This technique shows promise for advanced cell sorting and delivery systems.

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    Area of Science:

    • Biophysics
    • Microfluidics
    • Cellular Engineering

    Background:

    • Accurate manipulation of human red blood cells (RBCs) is crucial for various biomedical applications.
    • Existing methods often face challenges with cell stress and precise control in fluidic environments.

    Purpose of the Study:

    • To develop and validate a novel technique for manipulating human RBCs in hydrodynamic flows.
    • To minimize laser-induced stress and heating during cell manipulation.
    • To quantitatively correlate forces with cell deformation using computational fluid dynamics.

    Main Methods:

    • Utilized optical tweezers to trap and move microbead-attached RBCs in a liquid medium.
    • Employed computational fluid dynamics (CFD) to simulate flow-induced shear stress and cell deformation.
    • Operated under conditions minimizing laser heating and photon-induced stress.

    Main Results:

    • Successfully manipulated human RBCs at various speeds in hydrodynamic flows.
    • Demonstrated significantly minimized laser heating and photon-induced stress during cell trapping.
    • Quantitatively correlated applied forces with observed cell deformations via CFD simulations.

    Conclusions:

    • The developed optical tweezer technique enables precise manipulation of RBCs under physiological conditions.
    • This approach offers a foundation for next-generation cell sorting and targeted delivery systems.
    • Minimizing cellular stress during manipulation is key for preserving cell function.