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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
Human infection with G12 rotaviruses, Germany
Corinna Pietsch1, Uwe G Liebert
1Institute of Virology, Leipzig University, Leipzig, Germany. corinna.pietsch@medizin.uni-leipzig.de
Emerging Infectious Diseases
|October 1, 2009
Summary
Rotavirus group A G12 genotypes were detected in 1.5% of human rotavirus cases. Phylogenetic analysis confirmed these strains belong to the Wa-like genogroup, providing insights into rotavirus diversity.
Area of Science:
- Virology
- Molecular Biology
- Public Health
Background:
- Rotavirus is a leading cause of severe diarrheal disease in young children globally.
- Genotyping of rotavirus strains, particularly G and P types, is crucial for epidemiological surveillance and vaccine development.
Purpose of the Study:
- To detect and characterize Rotavirus group A G12 genotypes in human stool samples.
- To perform phylogenetic analysis of detected G12 strains to determine their genogroup affiliation.
Main Methods:
- Screening of 198 human rotavirus-positive stool samples for G12 genotypes using molecular methods.
- Genotyping of P types in conjunction with G types.
- Phylogenetic analysis of all 11 genomic RNA segments of identified strains.
Main Results:
- Rotavirus group A G12 genotypes were identified in 3 (1.5%) of the 198 samples.
- The G12P[6] genotype was found in 2 samples, and a mixed G3G12P[8] genotype in 1 sample.
- Phylogenetic analysis confirmed the Wa-like genogroup affiliation for all detected G12 strains.
Conclusions:
- G12 strains of Rotavirus group A are present, albeit at a low frequency, in the studied population.
- The identified G12 strains share common ancestry with the Wa-like genogroup.
- These findings contribute to understanding the genetic diversity and evolution of human rotavirus.
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