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Updated: Jun 20, 2026

A Protocol for Computer-Based Protein Structure and Function Prediction
Published on: November 3, 2011
EasyMIFS and SiteHound: a toolkit for the identification of ligand-binding sites in protein structures
Dario Ghersi1, Roberto Sanchez
1Department of Structural and Chemical Biology, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA.
Unlabelled:
SiteHound uses Molecular Interaction Fields (MIFs) produced by EasyMIFs to identify protein structure regions that show a high propensity for interaction with ligands. The type of binding site identified depends on the probe atom used in the MIF calculation. The input to EasyMIFs is a PDB file of a protein structure; the output MIF serves as input to SiteHound, which in turn produces a list of putative binding sites. Extensive testing of SiteHound for the detection of binding sites for drug-like molecules and phosphorylated ligands has been carried out.
Availability:
EasyMIFs and SiteHound executables for Linux, Mac OS X, and MS Windows operating systems are freely available for download from http://sitehound.sanchezlab.org/download.html.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
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