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Updated: Jun 20, 2026
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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Expression profiling of tetraploid mouse embryos in the developmental stages using a cDNA microarray analysis.
Jutaro Kawaguchi1, Kiyoshi Kano, Kunihiko Naito
1Laboratory of Applied Genetics, Graduate School of Agricultural and Life Science, University of Tokyo, Tokyo, Japan.
The Journal of Reproduction and Development
|October 1, 2009
Summary
Tetraploid embryos fail post-implantation. Gene expression analysis revealed significant downregulation of over 1,600 genes in tetraploid blastocysts, impacting cell division and potentially causing developmental failure.
Area of Science:
- Developmental Biology
- Genomics
- Mammalian Embryogenesis
Background:
- Tetraploid embryos develop to blastocyst stage but fail post-implantation.
- Understanding tetraploid embryo characteristics is crucial for developmental biology research.
Purpose of the Study:
- To investigate gene expression differences between diploid and tetraploid blastocysts.
- To identify genes and pathways affected in tetraploid embryos.
Main Methods:
- Production of tetraploid embryos via electrofusion.
- DNA microarray analysis of gene expression in hatched tetraploid and diploid blastocysts.
- Bioinformatics analysis using the VisuaL Annotation Display (VLAD) database.
Main Results:
- Most gene expression levels were similar between diploid and tetraploid blastocysts.
- Over 1,600 genes were downregulated and 2,800 genes were upregulated in tetraploid blastocysts.
- Suppressed pathways in tetraploid blastocysts included cell division, mitotic cell cycle, metabolic processes, and protein transport. Cyclin B1 (Ccnb1) was significantly downregulated.
Conclusions:
- A large population of downregulated genes characterizes tetraploid hatched blastocysts.
- Gene downregulation may be a primary factor defining the tetraploid phenotype.
- Altered cell cycle and metabolic processes contribute to tetraploid embryo developmental failure.
