Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell polarity control by an unconventional G-protein complex in bacteria.

Nature communications·2026
Same author

IglF mediates type VI secretion system spike assembly and promotes <i>Francisella</i> virulence.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Local peptidoglycan composition defines division site selection in Streptococcus pneumoniae.

Nature microbiology·2026
Same author

Streptococcus pneumoniae S protein activates PBP1a to regulate peptidoglycan remodelling and cell division.

Nature microbiology·2025
Same author

Visualization of peptidoglycan layer isolated from gliding diderm bacteria, <i>Flavobacterium johnsoniae</i> and <i>Myxococcus xanthus</i>, by quick-freeze deep-etch replica electron microscopy.

Biophysics and physicobiology·2025
Same author

Tad pili with adaptable tips mediate contact-dependent killing during bacterial predation.

Nature communications·2025

Related Experiment Video

Updated: Jun 19, 2026

Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System
09:12

Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System

Published on: January 16, 2019

A microscope automated fluidic system to study bacterial processes in real time.

Adrien Ducret1, Etienne Maisonneuve, Philippe Notareschi

  • 1Aix Marseille Université, Laboratoire de Chimie Bactérienne (UPR 9043), Institut de Microbiologie de la Méditerranée (IFR 88), CNRS, 31, Chemin Joseph Aiguier, Marseille, France.

Plos One
|October 1, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic device combining agar pads and flow chambers. This system enables real-time analysis of bacterial growth, cell cycle, and motility under dynamic conditions on agar surfaces.

More Related Videos

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
12:32

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Published on: November 25, 2020

Related Experiment Videos

Last Updated: Jun 19, 2026

Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System
09:12

Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System

Published on: January 16, 2019

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
12:32

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Published on: November 25, 2020

Area of Science:

  • Microbiology
  • Cell Biology
  • Bioengineering

Background:

  • Traditional time-lapse microscopy for bacterial studies uses agar pads, limiting dynamic environmental condition changes.
  • Existing fluidic approaches for eukaryotic cells are not easily adaptable to bacteria due to substrate specificity and microfluidic fabrication complexity.
  • Bacteria often grow and move on agar, making it a crucial experimental substrate.

Purpose of the Study:

  • To develop a novel microfluidic device for studying bacterial processes on agar surfaces.
  • To overcome the limitations of static agar pad experiments by enabling dynamic environmental perturbations.
  • To facilitate real-time, single-cell level analysis of bacterial behavior under controlled conditions.

Main Methods:

  • Designed a hybrid microfluidic device integrating a thin agar pad with a custom flow chamber.
  • Utilized the device to create controlled, dynamic environmental changes for bacterial cultures.
  • Applied time-lapse microscopy to observe bacterial growth, cell cycle progression, and motility.

Main Results:

  • The hybrid microfluidic system successfully supports bacterial growth and motility on agar.
  • Demonstrated the capability for real-time analysis of diverse bacterial biological processes.
  • Showcased the system's effectiveness in studying dynamic environmental impacts on single bacterial cells.

Conclusions:

  • The novel hybrid microfluidic device is an essential tool for studying bacterial processes on agar surfaces.
  • This system overcomes previous limitations, enabling dynamic perturbations and real-time observation.
  • Facilitates advanced single-cell level investigations of bacterial behavior and responses.