Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Infection following foot and ankle surgery : a subanalysis of data captured from the UK Foot and Ankle Thromboembolism (FATE) audit.

The bone & joint journal·2026

Same author

Postoperative pain after dental procedures: The National Dental Practice-Based Research Network observational study using an mobile health platform.

Journal of the American Dental Association (1939)·2026

Same author

Performance and Wellbeing Research Priorities in Premiership Women's Rugby: A Delphi Study Including Players and Staff.

European journal of sport science·2025

Same author

Design, manufacture and evaluation of an offloading 3D printed wheelchair cushion.

Disability and rehabilitation. Assistive technology·2025

Same author

Clinical and cost-effectiveness of flexor digitorum profundus (FDP) versus FDP and flexor digitorum superficialis (FDS) repair for complete zone 2 flexor tendon injuries (FLARE): protocol for a randomised controlled trial.

Trials·2025

Same author

The safety and efficacy of left atrial appendage closure devices in patients with non-traumatic intracranial hemorrhage.

Journal of the neurological sciences·2025

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

Published on: September 15, 2021

Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry.

David Holmes¹, David Pettigrew, Christian H Reccius

¹School of Electronics and Computing Science, University of Southampton, Highfield, Southampton, SO17 1BJ, UK. dh2@ecs.soton.ac.uk

|October 1, 2009

Summary

A novel microfluidic impedance cytometer offers rapid, label-free white blood cell differential counts. This point-of-care system shows high correlation with commercial equipment, promising accessible clinical blood analysis.

More Related Videos

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Published on: March 25, 2016

Related Experiment Videos

Last Updated: Jun 19, 2026

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

Published on: September 15, 2021

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Published on: March 25, 2016

Area of Science:

Biomedical Engineering
Analytical Chemistry
Hematology

Background:

Miniature, high-speed, label-free cell analysis systems are needed for routine clinical practice.
Current methods for full blood cell analysis can be slow, expensive, and complex.
Developing simple and rapid point-of-care diagnostic tools is a key goal in modern healthcare.

Purpose of the Study:

To develop and validate a microfluidic single cell impedance cytometer for white blood cell differential counting.
To demonstrate the feasibility of label-free cell analysis using impedance measurements.
To assess the clinical utility of the developed impedance microcytometer.

Main Methods:

A microfluidic chip with integrated micro-electrodes was designed to measure single cell impedance at two frequencies.
Human blood samples were treated to lyse erythrocytes, enabling analysis of white blood cells.
Cellular dielectric parameters were verified by correlating impedance signals with fluorescence from CD antibody-conjugated cells.

Main Results:

The microfluidic impedance cytometer successfully performed white blood cell differential counts in minutes.
Direct correlation between impedance signals and cell phenotype was achieved.
Patient sample testing demonstrated 95% correlation with commercial optical/Coulter blood analysis equipment.

Conclusions:

The developed impedance microcytometer is a promising technology for fast, inexpensive, and simple full blood cell analysis.
This system has significant potential for routine clinical practice and point-of-care blood analysis.
Label-free impedance cytometry offers a viable alternative to traditional cell analysis methods.