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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
J G Williams1, A R Kubelik, K J Livak
1Central Research and Development Department, E.I. du Pont de Nemours & Co., Inc, Wilmington, DE 19880.
Nucleic Acids Research
|November 25, 1990
Summary
A new method, Random Amplified Polymorphic DNA (RAPD) markers, uses random DNA amplification to identify genetic variations. These markers are inherited predictably and useful for creating genetic maps in diverse species.
Area of Science:
- Molecular genetics
- Genomics
- Population genetics
Background:
- Molecular genetic maps are crucial for understanding inheritance patterns.
- Traditional methods like RFLPs (Restriction Fragment Length Polymorphisms) rely on specific DNA sequence variations.
Purpose of the Study:
- To introduce a novel DNA polymorphism assay for genetic mapping.
- To establish a new type of genetic marker for broad applicability across species.
Main Methods:
- Utilizing single primers of arbitrary nucleotide sequence to amplify random DNA segments.
- Analyzing the amplification products for polymorphisms (presence or absence of DNA segments).
- Assessing the inheritance patterns of these polymorphisms through Mendelian analysis.
Main Results:
- The new assay detects DNA polymorphisms based on the amplification of random DNA segments.
- These polymorphisms, termed Random Amplified Polymorphic DNA (RAPD) markers, exhibit Mendelian inheritance.
- RAPD markers can be effectively used for constructing molecular genetic maps.
Conclusions:
- Random Amplified Polymorphic DNA (RAPD) markers provide a versatile and efficient tool for genetic mapping.
- This method offers a valuable alternative to existing techniques for genetic analysis in various species.
- RAPD markers facilitate the study of genetic diversity and inheritance in a wide range of organisms.