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Updated: Jun 19, 2026

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Published on: May 29, 2011
Entropic effects in channel-facilitated transport: interparticle interactions break the flux symmetry
Alexander M Berezhkovskii1, Mark A Pustovoit, Sergey M Bezrukov
1Mathematical and Statistical Computing Laboratory, Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Bethesda, Maryland 20892, USA.
Abstract:
We analyze transport through conical channels that is driven by the difference in particle concentrations on the two sides of the membrane. Because of the detailed balance, fluxes of noninteracting particles through the same channel, inserted into the membrane in two opposite orientations, are equal. We show that this flux symmetry is broken by particle-particle interactions so that one of the orientations can be much more efficient for transport under the same external conditions. The results are obtained analytically using a one-dimensional diffusion model and confirmed by three-dimensional Brownian dynamics simulations.
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