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Updated: Jun 19, 2026
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
Published on: January 7, 2019
Cellular targeting of engineered heterologous antigens is a determinant factor for bovine herpesvirus 4-based vaccine
Gaetano Donofrio1, Valentina Franceschi, Antonio Capocefalo
1Department of Animal Health, Sezione di Malattie Infettive degli Animali, Facoltà di Medicina Veterinaria, University of Parma, Parma, Italy. gaetano.donofrio@unipr.it
Abstract:
In a previous study, an apathogenic strain of bovine herpesvirus 4 (BoHV-4) cloned as a bacterial artificial chromosome and expressing a chimeric peptide (gE2/gD) as a secreted form was described. Recombinant virus-inoculated animals produced antibodies against bovine viral diarrhea virus (BVDV) gE2 and BoHV-1 gD. However, neutralizing antibodies were produced only against BVDV, not against BoHV-1. In the present work a recombinant BoHV-4 expressing a membrane-linked form of gE2/gD chimeric peptide was constructed, and inoculated rabbits produced serum-neutralizing antibodies against both BVDV and BoHV-1. Protein cell sorting and targeting are a very important issue when immunodominant antigens are engineered for recombinant virus vaccine development.
