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Updated: Jun 19, 2026

Dissection of the Endolymphatic Sac from Mice
Published on: March 29, 2021
Endolymphatic sac is involved in the regulation of hydrostatic pressure of cochlear endolymph
Ryuhei Inamoto1, Takenori Miyashita, Kosuke Akiyama
1Department of Otolaryngology, Faculty of Medicine, Kagawa University, Kagawa, Japan. ina@med.kagawa-u.ac.jp
Abstract:
To clarify the role of the endolymphatic sac (ES) in the regulation of endolymphatic pressure, the effects of isoproterenol, a beta-adrenergic receptor agonist, and acetazolamide, a potent carbonic anhydrase inhibitor, both of which decrease ES direct current potential on cochlear hydrostatic pressure, were examined in guinea pigs. When isoproterenol was applied intravenously, hydrostatic pressures of cochlear endolymph and perilymph were significantly increased with no change in endocochlear potential or the hydrostatic pressure of cerebrospinal fluid. Acetazolamide produced no marked change in the hydrostatic pressure of cochlear endolymph. In ears with an obstructed ES, the action of isoproterenol on the hydrostatic pressure of cochlear endolymph and perilymph was suppressed. These results suggest that the ES may regulate the hydrostatic pressure of the endolymphatic system via the action of the agents such as catecholamines on the ES.
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