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Spontaneous bursting activity in the developing entorhinal cortex.

Maxim G Sheroziya1, Oliver von Bohlen Und Halbach, Klaus Unsicker

  • 1Department of Neuroanatomy, University of Heidelberg, Interdisciplinary Center for Neurosciences, D-69120 Heidelberg, Germany.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|October 2, 2009
PubMed
Summary

Immature rat entorhinal cortex neurons exhibit prolonged bursting activity, influenced by calcium levels. This intrinsic bursting is crucial for the maturation of neuronal networks in the developing brain.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Computational Neuroscience

Background:

  • The developing nervous system exhibits periodic spontaneous activity essential for maturation.
  • The entorhinal cortex (EC) is vital for memory, but its early development is poorly understood.
  • Spontaneous activity in immature EC layer III neurons remains largely uncharacterized.

Purpose of the Study:

  • Investigate spontaneous field potential (fp) activity and intrinsic firing patterns in medial EC layer III neurons during early postnatal development.
  • Characterize the properties and underlying mechanisms of prolonged intrinsic bursting activity in these neurons.
  • Examine the developmental changes in spontaneous activity patterns within the immature EC.

Main Methods:

  • Electrophysiological recordings (field potentials and whole-cell patch-clamp) from rat medial EC layer III neurons in brain slices.

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  • Manipulation of extracellular calcium concentration ([Ca(2+)](o)) to assess its effect on bursting activity.
  • Pharmacological blockade and suppression of specific ion channels and currents (e.g., I(CAN), I(Nap)) using agents like BAPTA, Cd(2+), FFA, TTX, nifedipine, and riluzole.

    • Main Results:

    • A subset of immature EC layer III neurons displayed prolonged (2-20s) voltage-dependent intrinsic bursting.
    • Reduced [Ca(2+)](o) increased the incidence of prolonged bursting, particularly at early postnatal days (P5-P10).
    • Bursting activity was dependent on Ca(2+)-sensitive nonspecific cationic current (I(CAN)) and persistent Na(+) current (I(Nap)), and blocked by TTX, Cd(2+), and FFA.
    • Spontaneous glutamate receptor-mediated events were observed at P5-P7, transitioning to slow-wave network oscillations by P11-P13.

    Conclusions:

    • Prolonged intrinsic bursting is a key feature of developing medial EC layer III neurons.
    • This activity pattern is modulated by extracellular calcium and specific ion currents (I(CAN), I(Nap)).
    • The observed spontaneous activity likely plays a role in neuronal and network maturation within the developing entorhinal cortex.