Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[New perspective of the 2023 American Academy of Audiology position statement on early identification of cytomegalovirus in newborns].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2024

Same author

[Research progress of deafness induced by cytomegalovirus infection in mice].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2023

Same author

[Effects of FoxO6 on proliferation and invasion of colorectal cancer cells].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2020

Same author

[Periostin regulated by let-7/miR-98 family mediates the apoptosis and epithelial-mesenchymal transition of colon cancer].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2019

Same author

[The expression and functional mechanism of long non-coding RNA LINC00339 in colorectal cancer].

Zhonghua yi xue za zhi·2019

Same author

[Advances in chronic obstructive pulmonary disease and epigenetics].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2017

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Energy-transfer-assisted lasing from microdroplets seeded with fluorescent sol.

R L Armstrong, J G Xie, T E Ruekgauer

|October 2, 2009

Summary

Researchers observed lasing emission from microdroplets containing Fluorescein 548 dye and fluorescent sol. Energy transfer mechanisms, including Förster resonance energy transfer, were identified as key drivers for this microdroplet laser phenomenon.

More Related Videos

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Related Experiment Videos

Last Updated: Jun 19, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Area of Science:

Optics and Photonics
Materials Science
Physical Chemistry

Background:

Microdroplets can act as optical microcavities, supporting morphology-dependent resonances.
Fluorescent dyes and nanoparticles can exhibit lasing under optical pumping.
Energy transfer mechanisms are crucial for understanding light emission in complex systems.

Purpose of the Study:

To investigate lasing emission from liquid microdroplets containing Fluorescein 548 dye and fluorescent sol.
To elucidate the energy transfer mechanisms responsible for sol lasing.
To explore the influence of sol size and concentration on emission properties.

Main Methods:

Fabrication of liquid microdroplets containing Fluorescein 548 dye and submicrometer fluorescent sol in ethanol.
Optical pumping of microdroplets using a laser.
Spectroscopic analysis of emission wavelengths and intensities.
Systematic variation of pump laser intensity and sol concentration.

Main Results:

Observed lasing emission from both fluorescein dye and the seeded fluorescent sol.
Demonstrated that sol lasing can be excited by fluorescein emission or directly by the pump laser.
Found that sol emission is dependent on sol size, with smaller sol showing emission even without fluorescein lasing.
Identified emission wavelengths corresponding to morphology-dependent resonances of the microdroplets.

Conclusions:

Lasing in the sol is driven by Förster energy transfer or enhanced radiative transfer.
Microdroplet microcavities play a critical role in enhancing and directing the emission.
The findings highlight the potential of engineered microdroplets for novel laser sources.